Nucleotide Sequence of Rainbow Trout (Saho gairdneri) Ribosomal 5.8 S Ribonucleic Acid*

The nucleotide sequence of rainbow trout (Salmo gairdneri) 5.8 S rRNA was determined to be p(C)-A-A-C-U-C-UIO LO SO Um-A-G-C-G-G-U(m)-G-G-A-U-C-A-C-U-C-G-G-C-U-C-G-U411 51, G-C-G-U-C-G-A-U-G-A-A-G-A-A-C-G-C-A-G-C-G-C-U-A-G80 ill C-(~V)-G-C-G-A-G-A-A-C-U-A-A-U-G-JI-G-A-A-U-U-Gm-C-AX0 911 1,N, G-G-A-C-A-C-A-U-U-G-A-U-C-A-U-U-G-A-C-A-C-U-U-C-G1 LO lL,l A-A-C-G-C-A-C-U-U-U-G-C-G-G-C-C-C-C-A-G-G-G-G-U-U,:,,I ,111 150 C-C-U-C-C-U-G-G-G-G-C-U-A-C-G-C-C-U-G-U-C-U-G-A-G,w U-G-U-C-G-C-U-U. The major molecular species is 161 nucleotides long; the sequence differs in only eight nucleotides from that of Man. An estimate of the secondary structure retains essentially all of the features previously described in mammalian 5.8 S RNAs with several interesting variations. The very stable G-C-rich hairpin loop is extended by two additional hydrogen bond pairs; the A-U-rich loop is shorter; and a third 2’@-methylation occurs at residue 8 close to the under methylated U(m) residue at position 14. Comparisons with other known sequences indicate that in eukaryotes 5.8 S rRNA is more highly conserved than 5 S RNA species.